The 2016 Daytona 500, the 58th running of the event, was a NASCAR Sprint Cup Series race held on February 21, 2016, at Daytona International Speedway in Daytona Beach, Florida. Contested over 200 laps on the 2.5-mile (4.0 km) asphalt superspeedway, it was the first race of the 2016 NASCAR Sprint Cup Series season. Denny Hamlin won the race in a photo finish over Martin Truex Jr.. The top-five was rounded out by Kyle Busch, Kevin Harvick and Carl Edwards.

Chase Elliott won the pole for the race and led three laps before crashing out early in the race and finished 37th. Dale Earnhardt Jr., and Kyle Busch won the Duel races. Unlike Busch, Earnhardt Jr. crashed out with 29 laps to go and finished 36th.

Hamlin led a race high of 95 laps on his way to winning the race. There were six caution flag periods for 31 laps and 20 lead changes among 15 different drivers.

This was the 27th career victory for Hamlin, first of the season, first at Daytona International Speedway and fifth win at the track for Joe Gibbs Racing. He left Daytona Beach with a five-point lead over Truex Jr. Toyota left with an eight-point lead over Chevrolet in the manufacturer standings.

The Daytona 500 was carried by Fox Sports on the broadcast Fox network for the American television audience. The radio broadcast for the race was carried by the Motor Racing Network and Sirius XM NASCAR Radio.